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green fluorescent protein gfp tagged e coli bl21  (ATCC)


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    Structured Review

    ATCC green fluorescent protein gfp tagged e coli bl21
    Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing <t>E.</t> <t>coli</t> with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.
    Green Fluorescent Protein Gfp Tagged E Coli Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/green fluorescent protein gfp tagged e coli bl21/product/ATCC
    Average 94 stars, based on 36 article reviews
    green fluorescent protein gfp tagged e coli bl21 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "A digital plating platform for robust and versatile microbial detection and analysis"

    Article Title: A digital plating platform for robust and versatile microbial detection and analysis

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-11525-6

    Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing E. coli with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.
    Figure Legend Snippet: Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing E. coli with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.

    Techniques Used: Isolation, Expressing, Fluorescence, Incubation

    Enrichment and identification of bacteria in complex samples by coupling the DP with selective and differential medium. ( A ) Enrichment of S. aureus from a microbial mixture consisting of S. aureus and GFP-expressing E. coli by coupling the DP platform with a NaCl-based selective medium. (i) Representative bright-field, fluorescence, and merged microimages of microcolonies formed in two PicoArray devices after being covered with LB medium agar sheet and selective medium agar sheet (incubated at 37 ºC for 8 h), respectively. Initial concentrations of S. aureus and E. coli ~ 3 × 10 5 CFU/mL and ~ 5 × 10 4 CFU/mL (ii) Enumeration of the fluorescent and non-fluorescent microcolonies formed in the PicoArray devices with LB medium agar sheet and selective medium agar sheet, respectively. ( B ) Representative bright-field microimages of microcolonies formed in three PicoArray devices after being loaded with S. aureus , E. coli , and S. aureus / E. coli mixture and incubated with MCA sheets at 37 ºC for 8 h.
    Figure Legend Snippet: Enrichment and identification of bacteria in complex samples by coupling the DP with selective and differential medium. ( A ) Enrichment of S. aureus from a microbial mixture consisting of S. aureus and GFP-expressing E. coli by coupling the DP platform with a NaCl-based selective medium. (i) Representative bright-field, fluorescence, and merged microimages of microcolonies formed in two PicoArray devices after being covered with LB medium agar sheet and selective medium agar sheet (incubated at 37 ºC for 8 h), respectively. Initial concentrations of S. aureus and E. coli ~ 3 × 10 5 CFU/mL and ~ 5 × 10 4 CFU/mL (ii) Enumeration of the fluorescent and non-fluorescent microcolonies formed in the PicoArray devices with LB medium agar sheet and selective medium agar sheet, respectively. ( B ) Representative bright-field microimages of microcolonies formed in three PicoArray devices after being loaded with S. aureus , E. coli , and S. aureus / E. coli mixture and incubated with MCA sheets at 37 ºC for 8 h.

    Techniques Used: Bacteria, Expressing, Fluorescence, Incubation

    Phenotyping antibiotic resistance using the DP platform. ( A ) Representative bright-field microimages showing E. coli microcolonies grown in the PicoArray chips under different concentrations of ampicillin sodium after 6 h of incubation at 37 ºC. Initial concentration of E. coli ~ 1 × 10 5 CFU/mL. ( B ) Dose response curve for E. coli viability after treatment with different concentrations of ampicillin sodium. Calculation of MIC using a Gompertz function fit. Blue vertical dashed line shows the position of the MIC. Each test was performed in triplicate ( n = 3). Error bars represent standard deviation (SD). ( C ) Representative bright-field microimages showing the morphology changes of E. coli under antibiotic stress.
    Figure Legend Snippet: Phenotyping antibiotic resistance using the DP platform. ( A ) Representative bright-field microimages showing E. coli microcolonies grown in the PicoArray chips under different concentrations of ampicillin sodium after 6 h of incubation at 37 ºC. Initial concentration of E. coli ~ 1 × 10 5 CFU/mL. ( B ) Dose response curve for E. coli viability after treatment with different concentrations of ampicillin sodium. Calculation of MIC using a Gompertz function fit. Blue vertical dashed line shows the position of the MIC. Each test was performed in triplicate ( n = 3). Error bars represent standard deviation (SD). ( C ) Representative bright-field microimages showing the morphology changes of E. coli under antibiotic stress.

    Techniques Used: Incubation, Concentration Assay, Standard Deviation



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    ATCC green fluorescent protein gfp tagged e coli bl21
    Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing <t>E.</t> <t>coli</t> with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.
    Green Fluorescent Protein Gfp Tagged E Coli Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/green fluorescent protein gfp tagged e coli bl21/product/ATCC
    Average 94 stars, based on 1 article reviews
    green fluorescent protein gfp tagged e coli bl21 - by Bioz Stars, 2026-02
    94/100 stars
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    Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing E. coli with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.

    Journal: Scientific Reports

    Article Title: A digital plating platform for robust and versatile microbial detection and analysis

    doi: 10.1038/s41598-025-11525-6

    Figure Lengend Snippet: Isolation and cultivation of bacterial cells from a microbial mixture consisting of S. aureus and GFP-expressing E. coli with the DP platform. (A) Typical bright-field, fluorescence, and merged microimages of microcolonies grown in a PicoArray device after 8 h of incubation at 37 °C. Initial concentrations of S. aureus and E. coli ~ 6 × 10 4 CFU/mL. (B) Enumeration of S. aureus and GFP-expressing E. coli in the microbial mixture using the DP platform vs. the agar plating.

    Article Snippet: The bacterial species used in this work were Escherichia coli ( E. coli ) JM109, green fluorescent protein (GFP)-tagged E. coli BL21, Staphylococcus aureus ( S. aureus ) ATCC 43,300, and Salmonella enterica 14,028, which were purchased from China General Microbiological Culture Collection Center.

    Techniques: Isolation, Expressing, Fluorescence, Incubation

    Enrichment and identification of bacteria in complex samples by coupling the DP with selective and differential medium. ( A ) Enrichment of S. aureus from a microbial mixture consisting of S. aureus and GFP-expressing E. coli by coupling the DP platform with a NaCl-based selective medium. (i) Representative bright-field, fluorescence, and merged microimages of microcolonies formed in two PicoArray devices after being covered with LB medium agar sheet and selective medium agar sheet (incubated at 37 ºC for 8 h), respectively. Initial concentrations of S. aureus and E. coli ~ 3 × 10 5 CFU/mL and ~ 5 × 10 4 CFU/mL (ii) Enumeration of the fluorescent and non-fluorescent microcolonies formed in the PicoArray devices with LB medium agar sheet and selective medium agar sheet, respectively. ( B ) Representative bright-field microimages of microcolonies formed in three PicoArray devices after being loaded with S. aureus , E. coli , and S. aureus / E. coli mixture and incubated with MCA sheets at 37 ºC for 8 h.

    Journal: Scientific Reports

    Article Title: A digital plating platform for robust and versatile microbial detection and analysis

    doi: 10.1038/s41598-025-11525-6

    Figure Lengend Snippet: Enrichment and identification of bacteria in complex samples by coupling the DP with selective and differential medium. ( A ) Enrichment of S. aureus from a microbial mixture consisting of S. aureus and GFP-expressing E. coli by coupling the DP platform with a NaCl-based selective medium. (i) Representative bright-field, fluorescence, and merged microimages of microcolonies formed in two PicoArray devices after being covered with LB medium agar sheet and selective medium agar sheet (incubated at 37 ºC for 8 h), respectively. Initial concentrations of S. aureus and E. coli ~ 3 × 10 5 CFU/mL and ~ 5 × 10 4 CFU/mL (ii) Enumeration of the fluorescent and non-fluorescent microcolonies formed in the PicoArray devices with LB medium agar sheet and selective medium agar sheet, respectively. ( B ) Representative bright-field microimages of microcolonies formed in three PicoArray devices after being loaded with S. aureus , E. coli , and S. aureus / E. coli mixture and incubated with MCA sheets at 37 ºC for 8 h.

    Article Snippet: The bacterial species used in this work were Escherichia coli ( E. coli ) JM109, green fluorescent protein (GFP)-tagged E. coli BL21, Staphylococcus aureus ( S. aureus ) ATCC 43,300, and Salmonella enterica 14,028, which were purchased from China General Microbiological Culture Collection Center.

    Techniques: Bacteria, Expressing, Fluorescence, Incubation

    Phenotyping antibiotic resistance using the DP platform. ( A ) Representative bright-field microimages showing E. coli microcolonies grown in the PicoArray chips under different concentrations of ampicillin sodium after 6 h of incubation at 37 ºC. Initial concentration of E. coli ~ 1 × 10 5 CFU/mL. ( B ) Dose response curve for E. coli viability after treatment with different concentrations of ampicillin sodium. Calculation of MIC using a Gompertz function fit. Blue vertical dashed line shows the position of the MIC. Each test was performed in triplicate ( n = 3). Error bars represent standard deviation (SD). ( C ) Representative bright-field microimages showing the morphology changes of E. coli under antibiotic stress.

    Journal: Scientific Reports

    Article Title: A digital plating platform for robust and versatile microbial detection and analysis

    doi: 10.1038/s41598-025-11525-6

    Figure Lengend Snippet: Phenotyping antibiotic resistance using the DP platform. ( A ) Representative bright-field microimages showing E. coli microcolonies grown in the PicoArray chips under different concentrations of ampicillin sodium after 6 h of incubation at 37 ºC. Initial concentration of E. coli ~ 1 × 10 5 CFU/mL. ( B ) Dose response curve for E. coli viability after treatment with different concentrations of ampicillin sodium. Calculation of MIC using a Gompertz function fit. Blue vertical dashed line shows the position of the MIC. Each test was performed in triplicate ( n = 3). Error bars represent standard deviation (SD). ( C ) Representative bright-field microimages showing the morphology changes of E. coli under antibiotic stress.

    Article Snippet: The bacterial species used in this work were Escherichia coli ( E. coli ) JM109, green fluorescent protein (GFP)-tagged E. coli BL21, Staphylococcus aureus ( S. aureus ) ATCC 43,300, and Salmonella enterica 14,028, which were purchased from China General Microbiological Culture Collection Center.

    Techniques: Incubation, Concentration Assay, Standard Deviation